About
Molecular Assemblies, now operating under TriLink BioTechnologies, is a pioneer in enzymatic DNA synthesis. Their proprietary Fully Enzymatic Synthesis (FES)™ technology represents a fundamental shift away from decades-old chemical synthesis methods that plateau at 99.6% step-wise incorporation efficiency and are limited in oligo length and purity. FES achieves 99.9% step-wise incorporation efficiency, enabling the reliable production of DNA oligos up to 400 bases in length. The process operates at up to 70°C, which melts secondary structure and allows synthesis of traditionally difficult sequences including high/low GC content regions, long homopolymer stretches, and repetitive elements. Unlike chemical synthesis, FES operates at neutral pH, eliminating the harsh acids and bases that degrade product quality and create disposal challenges. An in-process purification step removes the need for expensive and time-consuming post-synthesis PAGE or HPLC purification. Their modular manufacturing platform supports frequent order batches with fast turnaround times and highly predictable first-time pass rates. Molecular Assemblies serves critical life sciences sectors including therapeutics, vaccine development, immunotherapy, agricultural biotechnology, and DNA nanotechnology, making it an essential partner for researchers requiring long, accurate, and pure synthetic DNA.
Key Features
- Fully Enzymatic Synthesis (FES)™: Proprietary enzyme-based DNA synthesis achieving 99.9% step-wise incorporation efficiency, surpassing the 99.6% ceiling of chemical synthesis.
- Long Oligos Up to 400 Bases: Produces DNA oligonucleotides up to 400 bases in length, far exceeding the practical limits of traditional chemical synthesis methods.
- In-Process Purification: Built-in purification step delivers high-purity DNA without the need for expensive and time-consuming post-synthesis PAGE or HPLC purification.
- Difficult Sequence Synthesis: Operates at up to 70°C to melt secondary structures, enabling reliable synthesis of high/low GC content, homopolymer runs, and repetitive sequences.
- Fast Order-to-Shipment Turnaround: Modular manufacturing platform allows frequent order batches with highly predictable first-time pass rates, delivering long oligos in days rather than weeks.
Use Cases
- Synthesizing long DNA oligos for therapeutic development, including gene therapy and antisense oligonucleotide drugs.
- Producing high-purity DNA sequences for vaccine research and mRNA vaccine development workflows.
- Generating accurate DNA templates for immunotherapy research, including CAR-T cell and checkpoint inhibitor studies.
- Creating synthetic DNA constructs for agricultural biotechnology applications such as crop trait engineering.
- Building complex DNA nanostructures for nanotechnology research requiring long and precise oligonucleotide sequences.
Pros
- Superior Purity and Accuracy: 99.9% step-wise incorporation efficiency results in higher-purity final product compared to chemical synthesis, reducing truncated species and errors.
- No Post-Synthesis Purification Needed: In-process purification eliminates costly PAGE or HPLC steps, saving time and reducing overall project costs.
- Environmentally Friendlier Process: Neutral pH synthesis avoids the harsh acids and bases of chemical synthesis, reducing hazardous waste and environmental impact.
- Handles Complex Sequences: High-temperature operation enables synthesis of difficult sequences that would otherwise fail or require special handling with chemical methods.
Cons
- Commercial Pricing Not Publicly Listed: Pricing requires direct contact with the sales team, which may slow down procurement for researchers on tight timelines.
- Niche Application Domain: Primarily relevant to life sciences and biotech research, limiting its utility to a specialized audience.
- Dependent on TriLink BioTechnologies Integration: As Molecular Assemblies is now part of TriLink BioTechnologies, service continuity and branding may evolve, requiring customers to adapt to new processes or contacts.
Frequently Asked Questions
What is Fully Enzymatic Synthesis (FES)™?
FES™ is Molecular Assemblies' proprietary enzyme-based DNA synthesis technology that achieves 99.9% step-wise incorporation efficiency, enabling production of long, pure DNA oligos without the limitations of traditional chemical synthesis.
How long can the DNA oligos be?
Using FES™ technology, Molecular Assemblies can produce DNA oligos up to 400 bases in length, significantly exceeding what is practical with chemical synthesis.
Is post-synthesis purification required?
No. An in-process purification step is built into the FES™ workflow, delivering highly pure DNA without the need for PAGE or HPLC purification after synthesis.
Can Molecular Assemblies synthesize difficult sequences?
Yes. The FES™ process operates at up to 70°C, which melts secondary structures and allows reliable synthesis of high/low GC content sequences, long homopolymers, and repetitive elements.
How does Molecular Assemblies relate to TriLink BioTechnologies?
Molecular Assemblies has been acquired by and is now part of TriLink BioTechnologies. Customers can contact TriLink directly for inquiries about FES™ products and services.
